Absorbance Detection
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β-lactamase reporter system for selecting high-producing yeast clones |
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A New Global Assay of Coagulation and Fibrinolysis |
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Adenovirus-mediated PTEN treatment combined with caffeine produces a synergistic therapeutic effect in colorectal cancer cells |
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Applications of a microplate reader in yeast physiology research |
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Evaluation of a Comprehensive Red Wine Phenolics Assay Using a Microplate Reader |
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Experimental Immune-Mediated Pancreatitis in Neonatally Thymectomized Mice Immunized with Carbonic Anhydrase II and Lactoferrin |
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Low-Intensity Laser Irradiation Improves the Mitochondrial Dysfunction of C2C12 Induced by Electrical Stimulation |
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Nitric oxide suppresses EPO-induced monocyte chemoattractant protein-1 in endothelial cells: implications for atherogenesis in chronic renal disease |
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Residue Leu-641 of Acetyl-CoA Synthetase is Critical for the Acetylation of Residue Lys-609 by the Protein Acetyltransferase Enzyme of Salmonella enterica |
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Serodiagnosis of Lyme Disease by Kinetic Enzyme-Linked Immunosorbent Assay Using Recombinant VlsE1 or Peptide Antigens of Borrelia burgdorferi Compared with 2-Tiered Testing Using Whole-Cell Lysates |
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Serum albumin leads to false-positive results in the XTT and the MTT assay |
Drug Discovery – HTS
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α Domain Deletion Converts Streptokinase into a Fibrin-dependent Plasminogen Activator through Mechanisms Akin to Staphylokinase and Tissue Plasminogen Activator |
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A human biotin acceptor domain allows site-specific conjugation of an enzyme to an antibody-avidin fusion protein for targeted drug delivery |
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A novel mutation of the cytochrome-b5 reductase gene in an Indian patient: the molecular basis of type I methemoglobinemia |
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A PDZ-binding Motif as a Critical Determinant of Rho Guanine Exchange Factor Function and Cell Phenotype (Synergy™ HT Multi-Detection Microplate Reader using BD Fluoroblok for measuring cell migration) |
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A receptor that mediates the post-mating switch in : Drosophila: reproductive behaviour |
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Anti-inflammatory and antioxidant activity of a medicinal tincture from Pedilanthus tithymaloides |
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Aspergillus fumigatus Variant with Decreased Susceptibility to Multiple Antifungals |
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Automatic method for determination of total antioxidant capacity using 2,2-diphenyl-1-picrylhydrazyl assay |
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Biochemical consequences of alginate encapsulation: A NMR study of insulin-secreting cells |
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Brain Region-Specific, Age-Related, Alterations in Mitochondrial Responses to Elevated Calcium |
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Cellular Delivery of MRI Contrast Agents |
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Chromosomally located gene fusions constructed in Acinetobacter sp. ADP1 for the detection of salicylate |
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Cisplatin Nephrotoxicity Is Mediated by Deoxyribonuclease |
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Cocaine-mediated enhancement of Tat toxicity in rat hippocampal cell cultures: The role of oxidative stress and D1 dopamine receptor |
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Cohesin mediates transcriptional insulation by CCCTC-binding factor |
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Comparative studies of Avipox-GM-CSF versus recombinant GM-CSF protein as immune adjuvants with different vaccine platforms |
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Components of intestinal epithelial hypoxia activate the virulence circuitry of Pseudomonas |
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Conjugated Linoleic Acid Inhibits Cell Proliferation through a p53-Dependent Mechanism: Effects on the Expression of G1-Restriction Points in Breast and Colon Cancer Cells |
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Cross-talk with Ca2+ Influx Does Not Underlie the Role of Extracellular Signal-regulated Kinases in Cytotoxic T Lymphocyte Lytic Granule Exocytosis |
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Cyclic strain increases fibroblast proliferation, matrix accumulation, and elastic modulus of fibroblast-seeded polyurethane constructs |
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Design, synthesis, and SAR analysis of cytotoxic sinapyl alcohol derivatives |
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Different effect of beta-carotene on proliferation of prostate cancer cells |
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Discovery of a novel protein tyrosine phosphatase-1B inhibitor, KR61639: potential development as an antihyperglycemic agent |
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Effects of cyclin-dependent kinase-5 activity on apoptosis and tau phosphorylation in immortalized mouse brain cortical cells |
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Effects of growth regulation on conditionally-transformed alginate-entrapped insulin secreting cell lines in vitro |
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Evaluation of angiotensin converting enzyme (ACE) and its homologue (ACE2) and neprilisyn and angiotension peptide metabolism |
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Furocoumarins from grapefruit juice and their effect on human CYP 3A4 and CYP 1B1 isoenzymes |
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Herpesvirus infection of ICAM-1-deficient mice |
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High Throughput Assay of Oxygen Radical Absorbance Capacity (ORAC) Using a Multichannel Liquid Handling System Coupled with a Microplate Fluorescence Reader in 96-Well Format |
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Highly active and selective endopeptidases with programmed substrate specificities |
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His tag effect on solubility of human proteins produced in Escherichia coli: a comparison between four expression vectors |
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Human monocytes differentiate into macrophages under the influence of human KPB-M15 conditioned medium |
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Human umbilical cord blood-derived f-macrophages retain pluripotentiality after thrombopoietin expansion |
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Hydrogen peroxide scavenging activity by non-steroidal anti-inflammatory drugs |
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Improved psychomotor performance in aged mice fed diet high in antioxidants is associated with reduced ex vivo brain interleukin-6 production |
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In vitro irradiation of basement membrane enhances the invasiveness of breast cancer cells |
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Inhibition of dipeptidyl peptidase IV by novel inhibitors with pyrazolidine scaffold |
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Inhibition of human neutrophil oxidative burst by pyrazolone derivatives |
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Interferon-γ and Tumor Necrosis Factor-α; Synergize to Induce Intestinal Epithelial Barrier Dysfunction by Up-Regulating Myosin Light Chain Kinase Expression |
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Invasiveness of breast cancer cells MDA-MB-231 through extracellular matrix is increased by the estradiol metabolite 4-hydroxyestradiol |
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Is prophylactic radiotherapy necessary asprevention of tumour seeding following thoracoscopy in malignant pleural mesothelioma (MPM)? |
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Isolation of highly purified DNA from low-abundance archived and stained cytological specimens |
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KR-62436, 6-{2-[2-(5-cyano-4,5-dihydropyrazol-1-yl)-2-oxoethylamino]ethylamino}nicotinonitrile, is a novel dipeptidyl peptidase-IV (DPP-IV) inhibitor with anti-hyperglycemic activity |
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Manganese potentiates lipopolysaccharide-induced expression of NOS2 in C6 glioma cells through mitochondrial-dependent activation of nuclear factor kappaB |
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Mathematical modeling of mesothelioma growth suggestsalternate response criteria |
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Minimization of between-well sample variance of antifungal activity using a high-throughput screening microplate bioassay |
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Multiple ligand binding sites on Aβ(1–40) fibrils |
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Muscle-specific expression of IGF-1 blocks angiotensin II–induced skeletal muscle wasting |
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Overview of the latest HT-7 experiments |
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Physical Interaction and functional coupling between ACDP4 and the intracellular ion chaperone Cox II, an implication of the role of ACDP4 in essential metal ion transport and homeostasis |
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Pindolol is a potent scavenger of reactive nitrogen species |
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PP2A-dependent transactivation of the cyclin A promoter by SV40 ST is mediated by a cell cycle-regulated E2F site |
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Proteasome or calpain inhibition does not alter cellular tau levels in neuroblastoma cells or primary neurons |
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Pulmonary Collectins Enhance Phagocytosis of Mycobacterium avium through Increased Activity of Mannose Receptor |
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Quantification of Carbamylated LDL in Human Sera by a New Sandwich ELISA |
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Quantification of green fluorescent protein fluorescence using real-time PCR thermal cycler |
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Ratiometric and nonratiometric Ca2+ indicators for the assessment of intracellular free Ca2+ in a breast cancer cell line using a fluorescence microplate reader |
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Spatial Distribution and Stability of the Eight Microbial Species of the Altered Schaedler Flora in the Mouse Gastrointestinal Tract |
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Specific phospholipid recognition by human immunodeficiency virus type-1 neutralizing anti-gp41 2F5 antibody |
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Synergistic antibody induction by antigen CD40 ligand fusion protein as improved immunogen |
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Synthesis of fluorescent oligonucleotide–EYFP conjugate: Towards supramolecular construction of semisynthetic biomolecular antennae |
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T-cell line for HIV drug screening using EGFP as a quantitative marker of HIV-1 replication |
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Telomere uncapping by the G-quadruplex ligand RHPS4 inhibits clonogenic tumour cell growth in vitro and in vivo consistent with a cancer stem cell targeting mechanism |
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The peroxisome proliferator-activated receptor γ ligand troglitazone induces apoptosis and p53 in rat granulosa cells |
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The polyglutamine neurodegenerative protein ataxin-3 binds polyubiquitylated proteins and has ubiquitin protease activity |
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The proteolytic activity of the paracaspase MALT1 is key in T cell activation |
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Use of forced vital capacity (FVC) measurement for the clinical management of malignant pleural mesothelioma (MPM): Observations from the pemetrexed plus cisplatin versus cisplatin study |
Fluorescence Detection
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A nonsynonymous SNP in human cytosolic sialidase in a small Asian population results in reduced enzyme activity: potential link with severe adverse reactions to oseltamivir |
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A PDZ-binding Motif as a Critical Determinant of Rho Guanine Exchange Factor Function and Cell Phenotype |
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ACE2 inhibition worsens glomerular injury in association with increased ACE expression in streptozotocin-induced diabetic mice |
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Actin Depolymerization Disrupts Tight Junctions via Caveolae-mediated Endocytosis |
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Activation of Kainate Receptors Sensitizes Oligodendrocytes to Complement Attack |
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Cigarette smoke inhibits macrophage sensing of Gram-negative bacteria and lipopolysaccharide: relative roles of nicotine and oxidant stress |
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Differential expression of DHHC9 in microsatellite stable and instable human colorectal cancer subgroups |
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L-Glutamine ameliorates acetaldehyde-induced increase in paracellular permeability in Caco-2 cell monolayer |
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Proapoptotic Effects of Caspase-1/Interleukin-Converting Enzyme Dominate in Myocardial Ischemia |
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Purinergic Receptor-Stimulated IP3-Mediated Ca2+ Release Enhances Neuroprotection by Increasing Astrocyte Mitochondrial Metabolism during Aging |
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Quantifying Cell Migration and Invasion |
General
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BioTek Instruments: Hybrid Solutions |
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Development of an Alu-based, QSY 7-labeled Primer PCR Method for Quantitation of Human DNA in Forensic Samples |
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Prep and Curation of Nucleic Acid Collections |
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Screening Facility, Harvard Medical School - Plate Readers |
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Townsend Letter - The Human Bloodprint |
Liquid Handling
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A Fluid Exchange of New Ideas |
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A Rational Approach to Monoclonal Antibody Development According to Predefined Assay Criteria |
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BioTek's Precision2000 |
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Cell Screening Core Facility, Cornell University |
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Development of an HTS FabD Inhibition Assay using a New 384-well Filter Plate to Screen Novel Antibiotics |
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Modular Semi-Automatic System for the Detection of PrPSc using the Prionics CheckLIA BSE Rapidtest |
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Multiplexing and Microassays |
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Screening Facility, Harvard Medical School - Liquid Handling |
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Screening Facility, Harvard Medical School - Plate Washers |
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Utilization of an Automated Triplex Assay |
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Validating a custom multiplex ELISA against individual commercial immunoassays using clinical samples |
Luminescence Detection
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Ionic Contacts at DnaK Substrate Binding Domain Involved in the Allosteric Regulation of Lid Dynamics |
